Liberation thermique d'une proteine recombinante dans un milieu de culture

Thermal release of recombinant protein into culture media

Abstract

The invention relates to a method for the isolation of a substantially pure polypeptide from a recombinant bacterial host by culturing the host under recombinant protein-producing conditions followed by heating the aqueous nutrient medium to 50-100°C for a time not to exceed 1 hour and recovering the substantially pure polypeptide so produced.
La présente invention se rapporte à un procédé pour l'isolation d'un polypeptide pratiquement pur contenu dans un hôte bactérien recombinant, par la culture dudit hôte dans des conditions de production d'une protéine recombinante, suivie du chauffage du milieu nutritif aqueux à une température de 50-100°C pendant une durée n'excédant pas une heure, puis de la récupération du polypeptide quasiment pur ainsi produit.

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Patent Citations (1)

    Publication numberPublication dateAssigneeTitle
    US-3585179-AJune 15, 1971Kyowa Hakko Kogyo KkProcess for extracting proteins from microorganisms

NO-Patent Citations (4)

    Title
    Biochimica et Biophysica ACTA, Vol. 38, issued 1960, A. GAREN and C. LEVINTHAL, a fine Structure Genetic and Chemical Study of the Enzyme Alkaline Phosphatase of E. Coli. pages 470-483. see especially page 473 last paragraph, continued on 474.
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    SCOPES, ROBERT K., "Protein Purification", 1982, Springer-Verlag, New York, pages 61-63.
    See also references of EP 0422124A4

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    US-5985304-ANovember 16, 1999Battelle Memorial InstituteBarrier preventing wood pest access to wooden structures
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